Wisdom Electronic Library

Genomics of Disease

2008-04-05T10:16:00.001+03:00

Product Details

Hardcover: 240 pages
Publisher: Springer; 1 edition (March 19, 2008)
Language: English
ISBN-10: 0387767223
ISBN-13: 978-0387767222
Product Dimensions: 9.3 x 6.2 x 0.7 inches

· Product Description

The title will develop from the 24th Stadler symposium. It will explore the general theme "GENOME EXPLOITATION: Data Mining the Genomes". The idea behind the theme is to discuss and illustrate how scientists are going to characterize and make use of the massive amount of information being accumulated about plant and animal genomes. The book will present a state-of-the-art picture on mining the Genome databases. This will be one of the few times that researchers in both plants and animals will be working together to create a seminal data resource.

Contents

Roles of Plant Hormones in Plant Resistance

and Susceptibility to Pathogens

Lionel Navarro, Rajendra Bari, Alexandre Seilaniantz, Adnane Nemri,

and Jonathan D.G. Jones

Canine Genetics Facilitates Understanding of Human Biology

Elaine A. Ostrander, Heidi G. Parker, and Nathan B. Sutter

Xanthomonas oryzae pv. oryzae AvrXA21 Activity Is Dependent

on a Type One Secretion System, Is Regulated by a Two-Component

Regulatory System that Responds to Cell Population Density,

and Is Conserved in Other Xanthomonas spp.

Sang-Won Lee, Sang-Wook Han, Laura E. Bartley, and Pamela C. Ronald

Unraveling the Genetic Mysteries of the Cat: New Discoveries

in Feline-Inherited Diseases and Traits

Leslie A. Lyons

Variation in Chicken Gene Structure and Expression Associated

with Food-Safety Pathogen Resistance: Integrated Approaches

to Salmonella Resistance

S.J. Lamont

Functional Genomics and Bioinformatics of the Phytophthora sojae

Soybean Interaction

Brett M. Tyler, Rays H.Y. Jiang, Lecong Zhou, Sucheta Tripathy,

Daolong Dou, Trudy Torto-Alalibo, Hua Li, Yongcai Mao, Bing Liu,

Miguel Vega-Sanchez, Santiago X. Mideros, Regina Hanlon, BrianM. Smith,

Konstantinos Krampis, Keying Ye, Steven St. Martin, Anne E. Dorrance,

Ina Hoeschele, and M.A. Saghai Maroof

Canine SINEs and Their Effects on Phenotypes of the Domestic Dog

Leigh Anne Clark, Jacquelyn M. Wahl, Christine A. Rees, George M. Strain,

Edward J. Cargill, Sharon L. Vanderlip, and Keith E. Murphy

Ovine Disease Resistance: Integrating Comparative and Functional

Genomics Approaches in a Genome Information-Poor Species

H.W. Raadsma, K.J. Fullard, N.M. Kingsford, E.T. Margawati,

E. Estuningsih, S. Widjayanti, Subandriyo, N. Clairoux, T.W. Spithill,

and D. Piedrafita

Integrating Genomics to Understand the Marek’s Disease

Virus – Chicken Host–Pathogen Interaction

Hans H. Cheng

Combining Genomic Tools to Dissect Multifactorial Virulence

in Pseudomonas aeruginosa

Daniel G. Lee, Jonathan M. Urbach, Gang Wu, Nicole T. Liberati,

Rhonda L. Feinbaum, and Frederick M. Ausubel

Genetic Dissection of the Interaction Between the Plant Pathogen

Xanthomonas campestris pv. vesicatoria and Its Host Plants

Ulla Bonas, Doreen G¨urlebeck, Daniela B¨uttner, Monique Egler,

Simone Hahn, Sabine Kay, Antje Kr¨uger, Christian Lorenz, Robert Szczesny,

and Frank Thieme

Structure and Function of RXLR Effectors

of Plant Pathogenic Oomycetes William Morgan, Jorunn Bos, Catherine Bruce, Minkyoung Lee,

Hsin-Yen Liu, Sang-Keun Oh, Jing Song, Joe Win, Carolyn Young,

and Sophien Kamoun

The Biotrophic Phase of Ustilago maydis: Novel Determinants

for Compatibility

Thomas Brefort, Kerstin Schipper, Gunther D¨ohlemann,

and Regine Kahmann

Virulence Evolution in Malaria

M.J. Mackinnon

The Ins and Outs of Host Recognition of Magnaporthe oryzae

Sally A. Leong

Index

Color Atlas of Genetics 3rd

2008-04-04T17:11:00.003+03:00

Product Details

# Paperback: 496 pages

# Publisher: Thieme Medical Publishers; 3rd edition (January 6, 2006)

# Language: English

# ISBN-10: 1588903362

# ISBN-13: 978-1588903365

American Journal of Medical Genetics

A remarkable achievement...concise but informative...No geneticist or
physician should be without a copy of this remarkable edition. --This
text refers to the Paperback edition.

Book Description

A remarkable achievement by a single author...concise but
informative...No geneticist or physician interested in genetic diseases
should be without a copy of this remarkable edition." --American
Journal of Medical Genetics understanding of genetics is a fundamental element of all medical and
scientific educational programs, across virtually all disciplines. And
the applications--and implications--of genetic research are at the
heart of current medical scientific debates. Completely updated and
revised, The Color Atlas of Genetics is an invaluable guide for
students of medicine and biology, clinicians, and anyone else
interested in this rapidly evolving field.

With more than 200
absorbing full-color plates concisely explained on facing pages, the
atlas offers readers an easy-to-use, yet remarkably detailed guide to
key molecular, theoretical, and medical aspects of genetics and
genomics. Brief descriptions of numerous genetic diseases are included,
with references for more detailed information.

Readers will find
that this incomparable book presents a comprehensive picture of the
field from its fascinating history to its most advanced applications.

Introduction

Chronology

Important Advances that Contributed

to the Development of Genetics

Part I. Fundamentals

Prologue

Taxonomy of Living Organisms:

The Tree of Life

Human Evolution

The Cell and Its Components

Molecular Basis of Genetics

Some Types of Chemical Bonds

Carbohydrates

Lipids (Fats)

Nucleotides and Nucleic Acids

Amino Acids

Proteins

DNA as a Carrier of Genetic

Information

DNA and Its Components

DNA Structure

Alternative DNA Structures

DNA Replication

The Flow of Genetic Information:

Transcription and Translation

Genes and Mutation

Genetic Code

Processing of RNA

DNA Amplification by Polymerase

Chain Reaction (PCR)

DNA Sequencing

Automated DNA Sequencing

Restriction Mapping

DNA Cloning

cDNA Cloning

DNA Libraries

Southern Blot Hybridization

Detection of Mutations

without Sequencing

DNA Polymorphism

Mutations

Mutations Due to Different Base

Modifications

Recombination

Transposition

Trinucleotide Repeat Expansion

DNA Repair

Xeroderma Pigmentosum

Prokaryotic Cells and Viruses

Bacteria in the Study of Genetics

Recombination in Bacteria

Bacteriophages

DNA Transfer between Cells

Classification of Viruses

Replication of Viruses

Retroviruses

Retrovirus Integration and

Transcription

Eukaryotic Cells

Cell Communication

Yeast: Eukaryotic Cells with a Diploid

and a Haploid Phase

Mating Type Determination in

Yeast Cells and Yeast Two-Hybrid

System

Cell Division: Mitosis

Meiosis in Germ Cells

Meiosis Prophase I

Formation of Gametes

Cell Cycle Control

Programmed Cell Death

Cell Culture

Mitochondrial Genetics

Mitochondria: Energy Conversion

Chloroplasts and Mitochondria

The Mitochondrial Genome

of Man

Mitochondrial Diseases

Formal Genetics

The Mendelian Traits

Segregation of Mendelian Traits

Independent Distribution of Two

Different Traits

Phenotype and Genotype

Segregation of Parental Genotypes

Monogenic Inheritance

Linkage and Recombination

Estimating Genetic Distance

Segregation Analysis with Linked

Genetic Markers

Linkage Analysis

Quantitative Differences in Genetic

Traits

Normal Distribution and Polygenic

Threshold Model

Distribution of Genes in a

Population

Hardy–Weinberg Equilibrium

Principle

Consanguinity and Inbreeding

Twins

Polymorphism

Biochemical Polymorphism

Differences in Geographical

Distribution of Some Alleles

Chromosomes

Chromosomes in Metaphase

Visible Functional Structures

of Chromosomes

Chromosome Organization

Functional Elements of

Chromosomes

DNA and Nucleosomes

DNA in Chromosomes

The Telomere

The Banding Patterns of

Human Chromosomes

Karyotypes of Man and Mouse

Preparation of Metaphase

Chromosomes for Analysis

Fluorescence In-Situ Hybridization

(FISH)

Aneuploidy

Chromosome Translocation

Structural Chromosomal

Aberrations

Multicolor FISH Identification of

Chromosomes

Comparative Genomic

Hybridization

Regulation of Gene Function

Ribosomes and Protein Assembly

Transcription

Prokaryotic Repressor and Activator:

the lac Operon

Genetic Control by Alternative RNA

Structure

Basic Mechanisms of

Gene Control

Regulation of Gene Expression in

Eukaryotes

DNA-Binding Proteins, I

DNA-Binding Proteins, II

RNA Interference (RNAi)

Targeted Gene Disruption

Epigenetic Modifications

DNA Methylation

Reversible Changes in Chromatin

Structure

Genomic Imprinting

Mammalian X Chromosome

Inactivation

Part II. Genomics

Genomics, the Study of the

Organization of Genomes

Gene Identification

Identification of Expressed DNA

Approaches to Genome Analysis

Genomes of Microorganisms

The Complete Sequence of the

Escherichia coli Genome

The Genome of a Multiresistant

Plasmid

Architecture of the Human Genome

The Human Genome Project

Genomic Structure of the Human X

and Y Chromosomes

Genome Analysis with DNA

Microarrays

Genome Scan and Array CGH

The Dynamic Genome: Mobile

Genetic Elements

Evolution of Genes and Genomes

Comparative Genomics

Part III. Genetics and

Medicine

Cell-to-Cell Interactions .

Intracellular Signal Transduction

Signal Transduction Pathways

TGF-_ and Wnt/_-Catenin Signaling

Pathways

The Hedgehog and TNF-α Signal

Transduction Pathways

The Notch/Delta Signaling Pathway

Neurotransmitter Receptors and Ion

Channels

Genetic Defects in Ion Channels:

LQT Syndromes

Chloride Channel Defects: Cystic

Fibrosis

Sensory Perception

Rhodopsin, a Photoreceptor

Mutations in Rhodopsin: Pigmentary

Retinal Degeneration

Color Vision

Auditory System

Odorant Receptors

Mammalian Taste Receptors

Genes in Embryonic Development

Genetic Determination of Embryonic

Development in Drosophila

Cell Lineage in a Nematode,

C. elegans

Developmental Genes in a Plant,

Arabidopsis

Immune System

Components of the Immune

System

Immunoglobulin Molecules

Genetic Diversity Generated by

Somatic Recombination

Mechanisms in Immunoglobulin

Gene Rearrangement

The T-Cell Receptor

Genes of the MHC Region

Evolution of the Immunoglobulin

Supergene Family

Hereditary Immunodeficiencies

Origins of Cancer

Genetic Causes of Cancer:

Background

Categories of Cancer Genes

The p53 Tumor Suppressor Gene

The APC Gene and Polyposis coli

Breast Cancer Susceptibility Genes

Retinoblastoma

The BCR/ABL Fusion Protein

in CML

Neurofibromatosis

Genomic Instability Diseases

Hemoglobin

Hemoglobin Genes

Sickle Cell Anemia

Mutations in Globin Genes

The Thalassemias

Hereditary Persistence of Fetal

Hemoglobin (HPFH)

DNA Analysis in Hemoglobin

Disorders

Lysosomes and Peroxisomes

Lysosomes

Diseases Due to Lysosomal Enzyme

Defects

Mucopolysaccharide Storage

Diseases

Peroxisomal Biogenesis Diseases

Cholesterol Metabolism

Cholesterol Biosynthesis Pathway

Distal Cholesterol Biosynthesis

Pathway

Familial Hypercholesterolemia

LDL Receptor Mutations

Homeostasis

Diabetes Mellitus

Protease Inhibitor α1-Antitrypsin

Blood Coagulation Factor VIII

(Hemophilia A)

VonWillebrand Bleeding Disease

Pharmacogenetics

Cytochrome P450 (CYP) Genes

Amino Acid Degradation and Urea

Cycle Disorders

Maintaining Cell and Tissue Shape

Cytoskeletal Proteins in

Erythrocytes

Hereditary Muscle Diseases

Duchenne Muscular Dystrophy

Collagen Molecules

Osteogenesis Imperfecta

Molecular Basis of Bone

Development

Sex Determination and Differentiation

Mammalian Sex Determination

Sex Differentiation

Disorders of Sexual Development

Congenital Adrenal Hyperplasia

Atypical Patterns of Genetic

Transmission

Diseases of Unstable Repeat

Expansion

Fragile X Syndrome

Karyotype–Phenotype Relationship

Autosomal Trisomies

Other Numerical Chromosomal

Deviation

Autosomal Deletion Syndromes

A Brief Guide to Genetic Diagnosis

Principles of Genetic Diagnostics

Gene and Stem Cell Therapy

Morbid Anatomy of the Human

Genome

Chromosomal Location of Human

Genetic Diseases

Chromosomal Location—Alphabetical

List

Appendix—Supplementary

Data

Glossary

Index

Principles and Technical Aspects of PCR Amplification

2008-04-04T15:54:00.002+03:00

Principles and Technical Aspects of PCR Amplification
by Elizabeth van Pelt-Verkuil (Author), Alexander van Belkum (Author), John P. Hays (Author)

Product Details
* Hardcover: 339 pages
* Publisher: Springer; 1 edition (April 2008)
* Language: English
* ISBN-10: 1402062400

Book Description
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes.
Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits their appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation.
This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control and quality assurance, variants and adaptations of the standard PCR protocol, quantitative PCR and in situ PCR. The reader of this manual will be excellently informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice.

Contents

Foreword

Chapter 1 The Polymerase Chain Reaction

1.1 An Overview of the PCR Process

1.2 Before PCR and Beyond

Chapter 2 A Brief Comparison Between In Vivo DNA Replication

and In Vitro PCR Amplifi cation

2.1 Nucleic Acid Targets

2.1.1 DNA

2.1.2 RNA

2.2 Target DNA Strand Separation and Primer Annealing

2.3 DNA Dependent DNA Polymerase and Oligonucleotide

Primers

2.4 Deoxyribonucleotides and Additional Factors

Chapter 3 The PCR in Practice

3.1 Brief Overview of PCR Requirements

3.1.1 The PCR Reaction Mix

3.1.2 The PCR Thermocycling Regime

3.1.3 Analysis of PCR Amplifi cation Products

3.1.4 Miscellaneous Considerations

Chapter 4 The Different Types and Varieties of Nucleic Acid

Target Molecules

4.1 General Features

4.2 A Brief Description of In Vivo DNA and RNA Targets

4.3 DNA Samples

4.3.1 DNA Isolation Procedures

4.3.2 Comments on Nucleic Acids in Specifi c

Sample Types

4.4 RNA Samples

4.4.1 Working Free of RNase Contamination

4.4.2 RNA Isolation for RT-PCR

4.5 Reverse Transcription and RT-PCR

4.5.1 cDNA Synthesis

4.5.2 cDNA Synthesis Using RACE

4.5.3 RNA Extraction and cDNA

Synthesis Controls

Chapter 5 PCR Primers

5.1 PCR Primer Design and Quality Requirements

5.1.1 Different Primer Species

5.2 Primer Hybridisation (Annealing)

5.3 Thermodynamic Approach of Tm Calculations

5.4 Primer Synthesis

5.5 Non-radioactive Primer Labelling

5.6 The Effect of Mismatches Between PCR

Primer and Target

5.7 Primer Concentration

Chapter 6 Deoxynucleotide Triphosphates and Buffer Components

6.1 Factors Affecting the Choice of dNTP Concentration

6.2 Modifi ed dNTPs and Their Applications

6.3 The PCR Buffer

6.3.1 Monovalent Ions

6.3.2 Magnesium Ions

Chapter 7 Taq and Other Thermostable DNA Polymerases

7.1 The Advantages and Disadvantages of Taq over

Klenow Fragment DNA Polymerase

7.2 Misincorporation of Nucleotides and Fidelity

of DNA Synthesis by Taq Polymerase

7.3 Taq DNA Polymerase and Its Modifi cations

7.4 Taq Polymerase Unit Defi nition and Working

Concentrations

7.5 Other Thermostable Polymerases

and Their Applications

7.6 Mixtures of Thermostable Polymerases

Chapter 8 Important Considerations for Typical, Quantitative

and Real-Time PCR Protocols

8.1 The Typical PCR Amplifi cation Protocol

8.1.1 Denaturation (Melting) of the Template DNA

8.1.2 Annealing (Hybridisation) of PCR Primers

8.1.3 Calculating the Primer Annealing

Temperature (Tm)

8.1.4 DNA Chain Extension/Elongation

8.1.5 PCR Cycle Number

8.1.6 The “Plateau Phase” and Final Stages of PCR

Thermocycling

8.1.7 PCR Sensitivity

8.2 Quantitative PCR Protocols

8.2.1 Quantitative PCR Controls

8.3 Real-Time PCR Protocols

8.4 RNA Extraction and Treatment

Chapter 9 Analysis of PCR Amplifi cation Products

9.1 Visualizing PCR Amplifi cation Products

9.1.1 Intercalating Chemical Dyes and Silver Ions

9.1.2 Fluorescent or Hapten Labelled Amplimers

9.2 Post-PCR Electrophoretic Analysis of Amplimers

9.2.1 Gel Electrophoresis Methodologies

9.2.2 Probe Hybridisation Methodologies

9.3 Real-Time Analysis of PCR Amplimers

9.3.1 In vitro Analysis Using Intercalating

Chemical Dyes

9.3.2 FRET Quenching Assays

9.3.3 TaqMan Probes

9.3.4 FRET Enhancement Reactions

9.4 Nucleic Acid Sequencing

9.4.1 DNA Sequencing Using Non-thermostable

DNA Polymerases

9.4.2 PCR Sequencing Using Thermostable

DNA Polymerases

9.4.3 The Fidelity of PCR Sequencing Reactions

Chapter 10 Ensuring PCR Quality – Laboratory Organisation, PCR

Optimization and Controls

10.1 The Primary Level of Quality Control – Laboratory

Organization and the Prevention of PCR

Contamination

10.1.1 Sources and Routes of Contamination

10.1.2 PCR Contamination Issues Within

Individual PCR Laboratories

10.1.3 Detecting and Preventing PCR

Contamination

10.2 The Secondary Level of PCR Quality Control – PCR

Design and Optimization

10.2.1 Extrinsic and Intrinsic Factors

10.2.2 The Developmental Steps Needed

to Achieve High Quality PCR Results

10.2.3 The Use of Positive and Negative Controls

in PCR Quality

10.2.4 Causes and Solutions for False Positive

and False Negative PCR Results

10.3 Quality Considerations Specifi c for RT-PCR

Methodologies

10.3.1 Problems Likely to Cause False Positive

Results in RT-PCR Assays

10.3.2 Problems Likely to Cause False Negative

Results in RT-PCR Assays

Chapter 11 Ensuring PCR Quality – Quality Criteria and Quality

Assurance

11.1 Quality Control Criteria and PCR

11.1.1 Sensitivity and Diagnostic Sensitivity

11.1.2 Specifi city and Diagnostic Specifi city

11.1.3 Reference and Threshold Values

11.1.4 The Predictive Value

11.1.5 Effi ciency

11.1.6 Error and Accuracy

11.1.7 Precision and Correctness

11.1.8 Defi ning the Analytical or Quantifi cation

Range and Sensitivity

11.1.9 Recovery, Reproducibility and Quality

Assurance

11.2 Quality Assurance and Multicenter Studies

Chapter 12 Variants and Adaptations of the Standard PCR Protocol

12.1 Generating Labelled PCR Amplimers for PCR

Product Visualization, DNA Probes and Cloning

12.2 Two-Step PCR Protocol

12.3 Booster PCR

12.4 Hot-Start and Time-Release PCR Protocols

12.5 Inverse PCR

12.6 Asymmetric PCR

12.7 PCR Mediated DNA Sequencing Strategies

12.7.1 Generating Single-Stranded DNA

for Sanger Sequencing Reactions

12.7.2 Classical Sanger Sequencing

of Single-Stranded PCR Products

12.7.3 Direct PCR Sequencing

12.7.4 Four-Tube Cycle Sequencing

12.7.5 One-Tube Cycle Sequencing

12.7.6 Diffi cult to Sequence Templates

12.8 Touchdown and Touch-Up PCR

12.9 Multiplex PCR

12.10 PCR Using Degenerate Primers

12.11 Repeat and Inter-repeat PCR

12.11.1 Repeat PCR

12.11.2 Inter-repeat PCR and Random Amplifi cation

of Polymorphic DNA (RAPD)

12.12 AFLP Fingerprinting

12.13 Base Excision Sequence Scanning (BESS-T-Scan)

for Mutation Detection

12.14 Differential Display RT-PCR (DD-PCR)

12.15 The Protein Truncation Test (PTT)

12.16 Methylation Specifi c PCR and PCR in the Detection

of Mutagens

12.17 Breakpoint PCR

12.18 Site Directed Mutagenesis by PCR

12.19 PCR Amplimers for Cloning and Expression

12.20 SAGE

12.21 PCR Inhibition by DNA Specifi c Antibiotics

and Mutagens

Chapter 13 In Situ PCR Amplifi cation (ISA) – Major Considerations,

Sample Processing and Applications

13.1 Tissue Processing – Nucleic Acid Fixation/Extraction

13.1.1 Fixation

13.1.2 Type of Nucleic Acid

13.1.3 Detrimental Effects of Various Fixatives

on Nucleic Acids

13.1.4 Effects of Tissue Processing Steps

(Decalcifi cation, Dehydration, Intermedium

Application, Embedding) and Storage

of Paraffi n Blocks

13.1.5 Effects of Histological and Histochemical

Staining

13.2 Differences in Approach for ISH, ISA and

Standard PCR

13.2.1 Different Types of Tissue Preparations .

13.2.2 DNA and RT-PCR on Paraffi n-Embedded

Tissue Sections

13.2.3 Improvement of PCR Effi ciency Using

Fixed Tissue Sections

13.3 An Introduction to In Situ Amplifi cation (ISA)

13.4 Considerations in the Development of ISA Protocols

13.4.1 IS-PCR or PCR-ISH

13.4.2 Diffusion of Nucleic Acids

13.4.3 The Correct Fixative

13.4.4 Damage Caused by Paraffi n Embedding

13.4.5 Detachment of Cells and Tissue Sections

13.4.6 Specimen Proteolysis

13.4.7 Acetylation and Other Forms of Tissue

Section Pre-treatment

13.4.8 Pre-treatment of Preparations for IS-PCR

13.4.9 Testing for Loss of Amplimers Due

to Leakage from Their Site of Production

13.4.10 Miscellaneous IS-PCR Considerations

13.4.11 Mispriming

13.4.12 Primer Independent Non-specifi c

DNA Synthesis

13.4.13 Evaporation of Reactants During IS-PCR/

Wet Hot Start Procedure

13.4.14 Cell Thickness and ISA

13.4.15 Choosing a Hybridisation Control

for Testing Amplimer Specifi city

13.4.16 Choice of the PCR Processor

13.4.17 Choice of the Final Detection Method

13.5 ISA Optimisation

13.6 ISA Controls

Index

Handbook of Human Immunology

2008-04-03T01:54:00.002+03:00

Handbook of Human Immunology, Second Edition
by Maurice R.G. O'Gorman (Editor), Albert D. Donnenberg (Editor)

Product Details
* Hardcover: 640 pages
* Publisher: CRC; 2 edition (February 15, 2008)
* Language: English
* ISBN-10: 0849319846

Book Description
Since the publication of the first edition of the Handbook of Human Immunology in 1997, major scientific achievements have elucidated our understanding of the complexities of the human immune system. This second edition compiles the major breakthroughs of the past decade including dramatic increases in the number of recognized clusters of differentiation on the surface of leukocytes and associated cells, establishment of an entire new chemokine and chemokine receptor nomenclature system, and discovery of more than 30 lymphokines. The text emphasizes clinically relevant immunological parameters, and clarifies the basic principles underlying immune system assays, as well as applications and interpretations of immune tests.

Contents

Preface

Editors

Contributors

Chapter 1 Overview of Immunity

James D. Folds

Chapter 2 Statistics of Immunological Testing

Albert D. Donnenberg

Chapter 3 Human Immunoglobulins

Robert G. Hamilton

Chapter 4 The Complement System

Patricia C. Giclas

Chapter 5 Cellular Immunology: Monitoring of Immune Therapies

Theresa L. Whiteside

Chapter 6 Understanding Clinical Flow Cytometry

Albert D. Donnenberg and Vera S. Donnenberg

Chapter 7 Leukemia and Lymphoma Immunophenotyping

and Cytogenetics

Maria A. Proytcheva

Chapter 8 Guidelines for the Use of Flow Cytometry in the

Management of Patients Infected with the Human

Immunodefi ciency Virus Type-1 and the Acquired

Immunodefi ciency Syndrome

Maurice R.G. O’Gorman

Chapter 9 Role of Flow Cytometry in the Diagnosis and Monitoring

of Primary Immunodefi ciency Disease

Maurice R.G. O’Gorman

Chapter 10 Detection and Characterization of the T-Cell

Receptor Repertoire

Gulbu Uzel

Chapter 11 Human Leukocyte Differentiation Antigens, Cluster

of Differentiation: Past, Present, and Future

Maurice R.G. O’Gorman

Chapter 12 Immunologic Diagnosis of Autoimmunity

Noel R. Rose

Chapter 13 Autoimmune and Immune-Mediated Diseases

of the Gastrointestinal Tract

Stefano Guandalini, Mala Setty, and Bana Jabri

Chapter 14 Serologic Testing for Infectious Diseases

John L. Schmitz

Chapter 15 Molecular Techniques Applied to Infectious Diseases

Jennifer S. Goodrich and Melissa B. Miller

Chapter 16 Cytokines: Regulators of Immune Responses and Key

Therapeutic Targets

Barbara Detrick, Chandrasekharam N. Nagineni,

and John J. Hooks

Chapter 17 Measuring Human Cytokines

Holden T. Maecker

Chapter 18 The Human Major Histocompatibility Complex and

DNA-Based Typing of Human Leukocyte Antigens

for Transplantation

Susana G. Marino, Andrés Jaramillo,

and Marcelo A. Fernلndez-Viٌa

Chapter 19 Relevance of Antibody Screening and Crossmatching

in Solid Organ, Hematopoietic Stem Cell Transplantation,

and Blood Transfusion

Chee L. Saw, Denise L. Heaney, Howard M. Gebel,

and Robert A. Bray

Chapter 20 Functional Assessment of Immunosuppression: Monitoring

Posttransplant Alloreactivity with Flow Cytometric Mixed

Lymphocyte Cocultures

Chethan Ashok Kumar, Ali Abdullah, Alison Logar,

Patrick Wilson, Anjan Talukdar, Nydia Chien,

Mandal Singh, and Rakesh Sindhi

Index

New Antibiotic Targets

2008-04-01T20:32:00.001+03:00

New Antibiotic Targets (Methods in Molecular Medicine)
By W. Scott Champney

Product Details
* Hardcover: 296 pages
* Publisher: Humana Press; 1 edition (January 15, 2008)
* Language: English
* ISBN-10: 1588299155

Book Description
A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In New Antibiotic Targets, W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies.
New Antibiotic Targets provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.

Contents

Preface .

Contributors

1 Biocomputational Strategies for Microbial Drug Target Identification

Kishore R. Sakharkar, Meena K. Sakharkar,

and Vincent T. K. Chow

2 Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase

IV Activities

L. Mark Fisher and Xiao-Su Pan

3 A Method to Assay Inhibitors of DNA Polymerase IIIC Activity

Michelle M. Butler and George E. Wright

4 Methods to Identify and Characterize Inhibitors of Bacterial RNA

Polymerase

A. Simon Lynch and Qun Du

5 Methods to Assay Inhibitors of tRNA Synthetase Activity

Dieter Beyer, Hein Peter Kroll, and Heike Brِtz-Oesterhelt

6 Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly

W. Scott Champney

7 Inhibition of Chaperone-Dependent Bacterial Ribosome Biogenesis

Abdalla Al Refaii and Jean-Hervé Alix

8 Assays for the Identification of Inhibitors Targeting Specific

Translational Steps

Letizia Brandi, John Dresios, and Claudio O. Gualerzi

9 SPARK: A New Peptidyl Transferase Activity Assay

Alexander S. Mankin and Norbert Polacek

10 High-Throughput Screening of Peptide Deformylase Inhibitors

Kiet T. Nguyen and Dehua Pei

11 A Method to Assay Penicillin-Binding Proteins

Michael J. Pucci and Thomas J. Dougherty

12 A Method to Assay Inhibitors of Lipopolysaccharide Synthesis

Marcy Hernick and Carol A. Fierke .

13 Methods for Assessing the Structure and Function of Cationic

Antimicrobial Peptides

Michelle Pate and Jack Blazyk

14 Flow Cytometry of Bacterial Membrane Potential and Permeability

Howard M. Shapiro

15 Bacterial Efflux Pump Inhibitors

Barbara J. Kamicker, Michael T. Sweeney, Frank Kaczmarek,

Fadia Dib-Hajj, Wenchi Shang, Kim Crimin, Joan Duignan,

and Thomas D. Gootz

16 Mycobacterium tuberculosis _-Ketoacyl Acyl Carrier Protein

Synthase III (mtFabH) Assay: Principles and Method

Sarbjot Sachdeva and Kevin A. Reynolds

17 Screening for Compounds That Affect the Interaction Between

Bacterial Two-Component Single Transduction Response

Regulator Protein and Cognate Promoter DNA

Matthew G. Erickson, Andrew T. Ulijasz, and Bernard Weisblum

18 The Activity of rRNA Resistance Methyltransferases Assessed

by MALDI Mass Spectrometry

Stephen Douthwaite, Rikke Lind Jensen, and Finn Kirpekar

19 Assays for _-Lactamase Activity and Inhibition

Thammaiah Viswanatha, Laura Marrone, Valerie Goodfellow,

and Gary I. Dmitrienko

20 Studies of Enzymes That Cause Resistance to Aminoglycoside

Antibiotics

Engin H. Serpersu, Can , and Edward Wright

Index

Microbial Diversity: Form and Function in Prokaryotes

2008-03-30T01:00:00.000+03:00

Microbial Diversity: Form and Function in Prokaryotes
by Oladele Ogunseitan (Author)

Product Details
Paperback: 312 pages
Publisher: Wiley-Blackwell (December 7, 2004)
Language: English
ISBN-10: 0632047089

Book Description
This book offers the first comprehensive, in-depth treatment of microbial diversity for undergraduate and graduate students. Using a global approach, Microbial Diversity illustrates the impact of microorganisms on ecological and Earth system phenomena.
Accompanied by a devoted website with resources for both instructors and students: www.blackwellpublishing.com/ogunseitan
Uses key ecological and global phenomena to show the continuity of microbial contribution.
Illustrates the importance of microbial diversity for the understanding of global physiochemical and biological processes.
Presents analyses of microscopic, culture, molecular, and phylogenetic systematic methods.
Shows the relevance of microbial diversity to global environmental problems, such as climate change and ozone depletion.
Features numerous illustrations, including over 60 4-color photographs of microbes.

CONTENTS

Foreword ix

Preface xiii

PART I CONCEPTS AND

METHODS 1

1 The concept of microbial species 3

Old and new challenges for assessing microbial

diversity 4

Traditional concepts of species 6

Typological species concept 6

Morphological species concept 7

Biological species concept 7

Evolutionary species concept 8

Other concepts 8

Species concepts for prokaryotes 10

Theoretical mechanisms of speciation 12

Anagenesis 12

Cladogenesis 13

Macroevolution theories 13

Species fusion theory 14

Gradual speciation 17

Microbial speciation 18

Conclusion: Emerging concepts and applications of

microbial diversity 19

Questions for further investigation 21

Humanized Mice

2008-03-30T00:56:00.001+03:00

Humanized Mice (Current Topics in Microbiology and Immunology)
by Tatsuji Nomura (Editor), Takeshi Watanabe (Editor), Sonoko Habu (Editor)

Product Details:
* Hardcover: 202 pages
* Publisher: Springer; 1 edition (April 2008)
* Language: English
* ISBN-10: 3540756469

Contents

Basic Concept of Development and Practical Application of

Animal Models for Human Disesases

T. Nomura, N. Tamaoki, A. Takakura, and H. Suemizu

Humanized SCID Mouse Models for Biomedical Research

T. Pearson, D. L. Greiner, and L. D. Shultz

NOD/Shi-scid IL2rg null (NOG) Mice More Appropriate for

Humanized Mouse Models

M. Ito, K. Kobayashi, and T. Nakahata

Humanizing Bone Marrow in Immune-Deficient Mice

K. Ando, Y. Muguruma, and T. Yahata

The Differentiative and Regenerative Properties of

Human Hematopoietic Stem/Progenitor cells in

NOD-SCID/IL2rg null Mice

F. Ishikawa, Y. Saito, S. Yoshida, M. Harada, and L. D. Shultz

Antigen-Specific Antibody Production of Human B Cells in

NOG Mice Reconstituted with the Human Immune System

R. Ito, M. Shiina, Y. Saito, Y. Tokuda, Y. Kametani, and S. Habu

Humanized Immune System (HIS) Mice as a Tool to Study Human

NK Cell Development

N. D. Huntington and J. P. Di Santo

Human T Cell Development and HIV Infection in

Human Hemato-Lymphoid System Mice

S. Baenziger, P. Ziegler, L. Mazzucchelli, L. Bronz,

R. F. Speck, and M. G. Manz

Humanized Mice for Human Retrovirus Infection

Y. Koyanagi, Y. Tanaka, M. Ito, and N. Yamamoto

Functional and Phenotypic Characterization of the

Humanized BLT Mouse Model

A. K. Wege, M. W. Melkus, P. W. Denton, J. D. Estes, and J. V. Garcia

Novel Metastasis Models of Human Cancer in NOG Mice

M. Nakamura and H. Suemizu

In Vivo Imaging in Humanized Mice.

H. Masuda, H. J. Okano, T. Maruyama, Y. Yoshimura,

H. Okano, and Y. Matsuzaki

Index

Plant Virus Evolution

2008-03-30T00:53:00.002+03:00

Plant Virus Evolution
by Marilyn J. Roossinck (Editor)

Product Details
* Hardcover: 224 pages
* Publisher: Springer; 1 edition (March 2008)
* Language: English
* ISBN-10: 3540757627

Book Description
This book provides a comprehensive look at the field of plant virus evolution. Individual chapters, written by experts in the field, cover plant virus ecology, emerging viruses, plant viruses that integrate into the host genome, population biology, evolutionary mechanisms and appropriate methods for analysis. It covers RNA viruses, DNA viruses, pararetroviruses and viroids. The book summarizes the recent advances in our understanding of genetic bottlenecks, mutation rates and RNA recombination. It presents a number of ideas that are thought-provoking and make excellent discussion points. It is rounded out by a description of the history of plant virus evolution studies, and the link between evolution and taxonomy. Plant Virus Evolution provides an invaluable resource for researchers, teachers and students in the fields of plant virology and virus evolution.

Contents

1 Questions and Concepts in Plant Virus Evolution: a Historical

Perspective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Fernando García-Arenal and Aurora Fraile

2 Community Ecology of Plant Viruses.

Alison G. Power

3 Emerging Plant Viruses: a Diversity of Mechanisms

and Opportunities

Maria R. Rojas and Robert L. Gilbertson

4 Evolution of Integrated Plant Viruses

Thomas Hohn, Katja R. Richert-Pöggeler, Christina Staginnus,

Glyn Harper, Trude Schwarzacher, Chee How Teo,

Pierre-Yves Teycheney, Marie-Line Iskra-Caruana, and Roger Hull

5 Viroids .

Robert A. Owens

6 Virus Populations, Mutation Rates and Frequencies

Justin S. Pita and Marilyn J. Roossinck

7 Genetic Bottlenecks

Akhtar Ali and Marilyn J. Roossinck

8 Recombination in Plant RNA Viruses

Peter D. Nagy

9 Symbiosis, Mutualism and Symbiogenesis

Marilyn J. Roossinck

10 Methods for Analyzing Viral Evolution

Marcos Pérez-Losada, Megan Porter, and Keith A. Crandall

11 Virus Evolution and Taxonomy

Anne-Lise Haenni

Index

The Interferons: Characterization and Application

2008-03-30T00:46:00.000+03:00

The Interferons: Characterization and Application
by Anthony Meager (Editor)

Product Details
* Hardcover: 460 pages
* Publisher: Wiley-VCH; 1 edition (May 1, 2006)
* Language: English
* ISBN-10: 3527311807

Book Description
At long last, the first book to cover all important areas of interferon science in one volume. Top scientists, including many pioneers in the field, highlight the role of interferons as research tools and as therapeutic agents in clinical applications. Edited by an experienced interferonologist, chapters include discussions of interferon genes, Type I, II and III IFNs, as well as their induction, production and purification, receptors actions, measuring IFN activities and anti-IFN antibodies, as well as the evolution of viral defense mechanisms.
For immunologists, cancer researchers, medicinal chemists, cell biologists, developmental biologists and the pharmaceutical industry.

Contents

Preface XIII

List of Contributors XV

Color Plates XIX

Section A Molecular Aspects, Introduction and Purification 1

1 Type I Interferons: Genetics and Structure 3

Shamith A. Samarajiwa, William Wilson and Paul J. Hertzog

1.1 Introduction 3

1.2 The Type I IFN Genetic Locus 4

1.3 Type I IFN Genes 7

1.3.1 IFN-a 7

1.3.2 IFN-b 7

1.3.3 IFN-o 8

1.3.4 IFN-k 8

1.3.5 IFN-e 9

1.3.6 IFN-z (Limitin) 9

1.3.7 IFN-t 9

1.3.8 IFN-d 10

1.3.9 IFN-n 10

1.4 Type I IFN Gene-regulatory Regions 11

1.5 Evolution of the Type I IFNs 15

1.5.1 Vertebrate IFN Genes 15

1.5.2 The Expansion and Divergence of the IFN Genes 20

1.6 Natural and Induced Mutations in IFN Genes 21

1.7 Secondary Structural Features of Type I IFNs 22

1.7.1 Conserved Amino Acid Residues 22

1.7.2 Post-translational Modifications of Type I IFNs 23

1.7.3 Conserved Cysteine Residues and Disulfide Bond Formation 24

1.8 The Structure of Type I IFNs 24

References 27

2 Activation of Interferon Gene Expression Through Toll-like Receptor-dependent

and -independent Pathways 35

Peyman Nakhaei, Suzanne Paz and John Hiscott

2.1 Introduction 35

2.2 IFN-b Gene Transcription 39

2.3 IRF Family Members 40

2.4 Role of IRFs in Virus-mediated IFN Activation 41

2.4.1 IRF-3 41

2.4.2 IRF-5 47

2.4.3 IRF-7 48

2.5 IFN Signaling Pathways 49

2.5.1 TLR-dependent Signaling to IFN Activation 49

2.5.1.1 TLR Overview 49

2.5.1.2 TLR-3 Signaling 51

2.5.1.3 TLR-4 Signaling 53

2.5.1.4 TLR-7 Signaling 57

2.5.1.5 TLR-9 Signaling 57

2.5.2 TLR-independent Signaling 58

2.5.2.1 Retinoic-inducible Gene (RIG)-I Signaling 58

2.5.2.2 Melanoma Differentiation-associated Gene-5 (mda-5) 59

2.6 Conclusions 61

References 61

3 Interferon Proteins: Structure, Production and Purification 73

Dimitris Platis and Graham R. Foster

3.1 Introduction 73

3.2 The Structure of Type I IFNs 73

3.3 Production and Purification of Type I IFNs 75

3.3.1 Leukocyte-derived IFN – First Steps in Producing Commercial IFN 75

3.3.2 Lymphoblastoid IFN – Towards more Reliable Supplies of IFN 76

3.3.3 Cloned Type I IFNs – An Inexhaustible Supply of Therapeutic

Material 76

3.4 Long-acting IFNs 78

3.5 Summary 79

References 80

4 Interferon-g: Gene and Protein Structure, Transcription Regulation, and

Actions 85

Ana M. Gamero, Deborah L. Hodge, David M. Reynolds, Maria Cecilia Rodriguez-

Galan, Mansour Mohamadzadeh and Howard A. Young

4.1 Introduction 85

4.2 IFN-g Gene Structure and Regulation 86

4.2.1 Transcriptional Regulation 86

4.2.2 Epigenetic Regulation 88

4.2.3 Post-transcriptional Regulation 90

4.3 IFN-g Signal Transduction 90

4.3.1 The JAK–STAT Signaling Pathway 91

4.3.2 Activation of Alternate Signaling Pathways 92

4.3.3 Regulation of IFN-g Signaling 93

4.4 IFN-g in Th Cell Development 95

4.4.1 Signaling Pathways Involved in T Cell Development 95

4.5 IFN-g and DCs 97

4.5.1 IFN-g and T Cell–DC Crosstalk 97

4.5.2 Signals through Toll-like Receptors (TLRs) Activate DCs and Influence

IFN-g Expression 99

4.6 IFN-g – Role in Tumor Development and Growth 100

4.6.1 IFN-g in Tumor Growth and Survival 100

4.6.2 Inhibition of Angiogenesis by IFN-g 101

4.6.3 Role of IFN-g in Promoting Immune Responses against Tumors 102

4.7 Summary 103

References 104

5 Interferon and Related Receptors 113

Sidney Pestka and Christopher D. Krause

5.1 Introduction 113

5.2 IFNs and IFN-like Molecules in Brief 113

5.3 The Receptors 114

5.3.1 Receptor Nomenclature 115

5.4 The Type I IFN Receptor 116

5.4.1 Discovery of the Type I Receptor Complex 117

5.4.2 Diversity of the Interaction of Type I IFNs with the Receptor 119

5.5 The Type II IFN (IFN-g) Receptor 121

5.5.1 Chromosomal Localization of the IFN-g Receptor Ligand-binding Chain

and Discovery of Two Chains Required for Activity 121

5.5.2 The IFN-gR1 Chain 123

5.5.3 The Second Receptor Chain (IFN-gR2) 123

5.5.4 The Functional IFN-gR Complex 124

5.5.4.1 Specificity of Ligand Binding 124

5.5.4.2 Specificity of the Interactions of the Two Chains 124

5.5.5 Specificity of Signal Transduction 124

5.5.6 Receptor Structure 125

5.5.7 Preassembly of the Receptor Complex 126

5.6 The IL-28R1 and -10R2 Receptor Complex 127

5.7 Overview of Multichain Receptors 128

5.8 Global Summary 128

References 129

6 Type III Interferons: The Interferon-l Family 141

Sergei V. Kotenko and Raymond P. Donnelly

6.1 Introduction 141

6.2 The Class II Cytokine Receptor Family (CRF2) and their Ligands 142

6.3 Genomic Structure 145

6.4 Receptor Complex and Signaling 148

6.5 Biological Activities 152

6.6 The Murine IFN-l Antiviral System 154

6.7 Evolution of the IFN Family 156

6.8 Therapeutic Potential 157

6.9 Conclusions 158

References 158

Section B Biological Properties 165

7 Biological Actions of Type I Interferons 167

Melissa M. Brierley, Jyothi Kumaran and Eleanor N. Fish

7.1 Introduction 167

7.2 Sources of Type I IFN Production and Secretion 167

7.3 Type I IFN Interactions with the Receptor Complex 168

7.3.1 Structure and Functional Regions of Type I IFNs 168

7.3.2 IFN Domains Mediating Interactions with IFNAR-2 168

7.3.3 IFNAR-2 Domains Mediating Interactions with IFNs 170

7.3.4 IFN-a Interaction with IFNAR-2 172

7.3.5 IFN-b Interaction with IFNAR-2 173

7.3.6 Type I IFN Interactions with IFNAR-1 174

7.3.7 IFNAR-1 Receptor Interaction with Glycosphingolipids 174

7.3.8 Residues in Type I IFNs that Mediate Biological Responses 175

7.4 Type I IFN-induced Signaling Cascades 175

7.4.1 The JAK–STAT (Signal Transducers and Activators of Transcription)

Pathway 175

7.4.1.1 The Importance of STAT-1 and -2 in Type I IFN Signaling 178

7.4.2 Other IFN-inducible Signaling Cascades 179

7.4.2.1 The CrkL Pathway 179

7.4.2.2 The IRS Pathway 179

7.4.2.3 The p38 Mitogen-activated Protein Kinases (MAPK) Pathway 181

7.4.2.4 The Vav Protooncogene and IFN Signaling 182

7.4.2.5 The Protein Kinase C (PKC) Family and IFN Signaling 182

7.5 IFN-inducible Biological Responses 183

7.5.1 IFN-inducible Antiviral Responses 183

7.5.1.1 PKR 186

7.5.1.2 20–50-OAS/RNase L 186

7.5.1.3 Mx 187

7.5.1.4 Other IFN-inducible Antiviral Effectors 188

7.5.2 IFN-inducible Growth-inhibitory Responses 188

7.5.3 IFN-inducible Immunomodulation 190

7.6 Summary 192

References 193

8 Interferons and Apoptosis – Recent Developments 207

Michael J. Clemens and Ian W. Jeffrey

8.1 Introduction 207

8.2 The Role of IFN-regulated Genes in the Control of Apoptosis 209

8.2.1 Proapoptotic Genes Induced by IFNs 209

8.2.2 p53 and IFN-induced Apoptosis 209

8.2.3 The Ribonuclease (RNase) L System 210

8.3 The Protein Kinase PKR and the Phosphorylation of Polypeptide Chain

Initiation Factor eIF2a 212

8.3.1 Regulation of Apoptosis by PKR 212

8.3.2 The Role of eIF2a Phosphorylation 212

8.4 IFNs and the Apoptotic Effects of TRAIL 215

8.4.1 TRAIL Induction 215

8.4.2 TRAIL Activity 215

8.5 Signal Transduction Pathways for IFN-mediated Effects on

Apoptosis 218

8.6 The Antiapoptotic Effects of IFNs 219

8.7 Conclusions 220

Acknowledgments 220

References 221

9 Viral Defense Mechanisms against Interferon 227

Santanu Bose and Amiya K. Banerjee

9.1 Introduction 227

9.2 Innate Immune Antiviral Defense Mechanisms of Host Cells 227

9.3 Evasion of IFN-mediated Antiviral Response 231

9.3.1 Nonsegmented Negative-strand RNA Viruses 232

9.3.1.1 Paramyxoviruses 232

9.3.1.2 Filovirus 242

9.3.1.3 Rhabdovirus 242

9.3.2 Segmented Negative-strand RNA Viruses 242

9.3.2.1 Orthomyxovirus 243

9.3.2.2 Bunyaviruses 243

9.3.3 Positive-sense ssRNA Viruses 244

9.3.3.1 Picornaviruses 244

9.3.3.2 Flaviviruses 244

9.3.3.3 Coronavirus 246

9.3.4 dsRNA Viruses 247

9.3.5 RNA and DNA Reverse-transcribing Viruses 247

9.3.5.1 Retrovirus 247

9.3.5.2 Hepadnavirus 248

9.3.6 dsDNA Viruses 248

9.3.6.1 Adenovirus 248

9.3.6.2 Poxvirus 248

9.3.6.3 Herpesvirus 250

9.3.6.4 Papillomavirus 252

9.4 Concluding Remarks 253

References 254

Section C Clinical Applications 275

10 Overview of Clinical Applications of Type I Interferons 277

Frank Mu¨ller

10.1 Introduction 277

10.2 Biological Effects 277

10.3 Type I IFN Products Currently Available or Under Development 278

10.4 Pharmacokinetics 284

10.4.1 IFN-a 284

10.4.2 IFN-b 285

10.5 Clinical Applications of Type I Interferons 285

10.5.1 Chronic hepatitis B (CHB) 286

10.5.2 Chronic hepatitis C (CHC) 288

10.5.3 Chronic hepatitis D (CHD) 290

10.5.4 Hairy Cell Leukemia (HCL) 290

10.5.5 Renal Cell Carcinoma (RCC) 291

10.5.6 Basal Cell Carcinoma (BCC) 292

10.5.7 Malignant Melanoma 292

10.5.8 Kaposi Sarcoma (KS) 292

10.5.9 Multiple Myeloma 293

10.5.10 Chronic myelogenous leukemia (CML) 294

10.5.11 Non-Hodgkinis lymphoma (NHL) 295

10.5.12 Laryngeal Papillomatosis 295

10.5.13 Mycosis Fungoides (MF) 296

10.5.14 Condyloma Acuminata 296

10.5.15 Multiple Sclerosis (MS) 297

10.6 IFN Toxicity 298

10.7 Type I IFNs in the Future 299

References 300

11 Clinical Applications of Interferon-g 309

Christine W. Czarniecki and Gerald Sonnenfeld

11.1 Introduction 309

11.2 IFN-g – The Molecule 311

11.3 FDA-approved Indications: Established Benefit and Risks 311

11.3.1 Chronic Granulomatous Disease (CGD) 311

11.3.2 Osteopetrosis 313

11.3.3 Adverse Reactions 314

11.4 Infectious Diseases 314

11.4.1 Mycobacterial Infection 314

11.4.1.1 Leprosy 314

11.4.1.2 Mycobacterium avium Infection 315

11.4.1.3 Tuberculosis (TB) 315

11.4.2 Leishmaniasis 317

11.4.3 Opportunistic Infections in HIV Disease 317

11.5 Infection Following Serious Trauma 318

11.6 Atopic Dermatitis (AD) 321

11.7 Idiopathic Pulmonary Fibrosis (IPF) 322

11.8 Systemic Sclerosis (SSc) 326

11.9 Radiation-induced Fibrosis 326

11.10 Chronic Hepatitis 327

11.11 Oncology Indications: Ovarian Cancer 329

11.12 Conclusions 330

References 331

Section D Measurement of Interferons and Anti-Interferons 337

12 Measurement of Interferon Activities 339

Tony Meager

12.1 Introduction 339

12.2 The IFNs: Mechanisms of Action, Protein Induction and Biological

Activities 340

12.2.1 Mechanisms of Action 340

12.2.2 Protein Induction 342

12.2.3 Biological Activities 344

12.2.3.1 Antiviral Activity 344

12.2.3.2 Antiproliferative Activity 344

12.2.3.3 Immunomodulatory Activity 345

12.3 Measurement of Biological Activities of IFNs 345

12.3.1 General Considerations 345

12.3.2 Biological Standards for IFNs 346

12.3.3 Practical Considerations for IFN Preparations 348

12.3.4 Antiviral Assays 349

12.3.5 Antiproliferative Assays 351

12.3.6 IFN-inducible Protein Assays 354

12.3.6.1 Bioimmunoassays 354

12.3.6.2 Enzyme Expression and Reporter Gene Assays 356

12.3.7 Assays Based on Intracellular Signaling Intermediates 361

12.3.8 Assay Design and Data Analysis 363

12.4 Regulatory Landscape 365

Acknowledgments 366

References 366

13 The Development and Measurement of Antibodies to Interferon 375

Sidney E. Grossberg and Yoshimi Kawade

13.1 Introductory Perspective 375

13.1.1 Immunological Perspective 375

13.1.2 Antibodies to Self-antigens 376

13.2 NAbs 377

13.2.1 Neutralization Bioassay Design 378

13.2.1.1 The Constant IFN Method 379

13.2.1.2 The Constant Antibody Method 380

13.2.2 Theoretical Analyses 382

13.2.3 Experimental Analyses 383

13.2.4 Standardization and the Reporting of Neutralization Results 383

13.2.5 A Solution to the Problem of NAb Unitage 385

13.3 Immunoassays for Non-NAbs 387

13.4 Epitope Analysis 388

13.5 Development of Antibodies during IFN Therapy 390

13.5.1 Antibodies to IFN-a 391

13.5.2 Antibodies to IFN-b 392

13.6 Summary 394

References 395

Index 401

Bacterial Biofilms

2008-03-30T00:41:00.002+03:00

Bacterial Biofilms (Current Topics in Microbiology and Immunology)
by Tony Romeo (Editor)

Product Details:
* Hardcover: 308 pages
* Publisher: Springer; 1 edition (February 29, 2008)
* Language: English
* ISBN-10: 3540754172

Book Description
Throughout the biological world, bacteria thrive predominantly in surface attached, matrix enclosed, multicellular communities or biofilms, as opposed to isolated planktonic cells. This choice of lifestyle is not trivial, as it involves major shifts in the use of genetic information and cellular energy, and has profound consequences for bacterial physiology and survival. Growth within a biofilm can thwart immune function and antibiotic therapy and thereby complicate the treatment of infectious diseases, especially chronic and foreign device-associated infections. Modern studies of many important biofilms have advanced well beyond the descriptive stage, and have begun to provide molecular details of the structural, biochemical and genetic processes that drive biofilm formation and its dispersion. There is much diversity in the details of biofilm development among various species, but there are also commonalities. In most species, environmental and nutritional conditions greatly influence biofilm development. Similar kinds of adhesive molecules often promote biofilm formation in diverse species. Signaling and regulatory processes that drive biofilm development are often conserved, especially among related bacteria. Knowledge of such processes holds great promise for efforts to control biofilm growth and combat biofilm-associated infections.

Contents

Biofilm Development with an Emphasis on Bacillus subtilis

K. P. Lemon, A. M. Earl, H. C. Vlamakis,

C. Aguilar, and R. Kolter

Physiology of Microbes in Biofilms

A. M. Spormann

Environmental Influences on Biofilm Development

C. C. Goller and T. Romeo

Quorum Sensing and Microbial Biofilms

Y. Irie and M. R. Parsek

Innate and Induced Resistance Mechanisms of

Bacterial Biofilms.

G. G. Anderson and G. A. O’Toole

Multidrug Tolerance of Biofilms and Persister Cells.

K. Lewis

Biofilms on Central Venous Catheters: Is Eradication Possible?

R. M. Donlan

Role of Bacterial Biofilms in Urinary Tract Infections

J. K. Hatt and P. N. Rather

Shifting Paradigms in Pseudomonas aeruginosa Biofilm Research

A. H. Tart and D. J. Wozniak

Staphylococcal Biofilms

M. Otto

Yersinia pestis Biofilm in the Flea Vector and Its Role in the

Transmission of Plague

B. J. Hinnebusch and D. L. Erickson

Escherichia coli Biofilms

C. Beloin, A. Roux, and J.-M. Ghigo

Index

Antimicrobial Resistance: Problem Pathogens and Clinical Countermeasures

2008-03-30T00:37:00.000+03:00

Antimicrobial Resistance: Problem Pathogens and Clinical Countermeasures (Infectious Disease and Therapy)
by Jr., Robert C. Owens (Editor), Ebbing Lautenbach (Editor)

Product Details
* Hardcover: 520 pages
* Publisher: Informa Healthcare; 1 edition (December 22, 2007)
* Language: English
* ISBN-10: 0824729412

Book Description
This publication provides a state-of-the-art overview of key issues related to antimicrobial resistance, including a focus on key pathogens causing common healthcare-associated and community-acquired infections. The epidemiology and therapeutic considerations of these antimicrobial resistant organisms are discussed, as well as the clinical and health economic impact of infections caused by them. This progressive reference also provides a dedicated section covering the clinical programmatic strategies used to minimize the growing antimicrobial resistance problem, including practical information related to interventional concepts and their implementation. In addition to antimicrobial resistance in the context of traditionally discussed problematic bacterial pathogens, emerging data related to clinically important fungal pathogens and Clostridium difficile are also covered.

Contents

Foreword Robert C. Moellering, Jr. v

Preface ix

Contributors xv

Part I: Resistance Concerns for the Practicing Clinician:

General Considerations

1. The Attributable Costs of Resistant Infections in Hospital Settings:

Economic Theory and Application 1

R. Douglas Scott II and Rebecca R. Roberts

2. Benchmarking: Its Utility in the Fight Against Antibacterial Resistance 25

Sujata M. Bhavnani and Glenn S. Tillotson

3. Antibiotic Resistance: Opportunity or Obstacle for the

Pharmaceutical Industry? 39

Glenn S. Tillotson

4. Understanding Studies of Resistant Organisms: Focus on

Epidemiological Methods 61

Ebbing Lautenbach

Part II: Contemporary Antimicrobial Resistance Issues: Gram-Positive,

Gram-Negative, and Miscellaneous Pathogens

5. Staphylococcus aureus: Resistance Update and Treatment Options 75

Pamela A. Moise and George Sakoulas

6. Enterococci: Resistance Update and Treatment Options 89

Peter K. Linden

7. Streptococcus pneumoniae: Resistance Update and Treatment Options 111

Nicole Akar, William R. Bishai, and Sanjay K. Jain

8. Acinetobacter Species: Resistance Update and Treatment Options 125

Lisa L. Maragakis and Trish M. Perl

9. Pseudomonas aeruginosa: An Understanding of Resistance Issues 149

Karen Lolans, Maria Virginia Villegas, and John P. Quinn

10. Problematic Beta-Lactamases: An Update 169

Marion S. Helfand and Louis B. Rice

11. Clostridium difficile-Infection: Overview and Update with a Focus on Antimicrobial

Resistance as a Risk Factor 183

Robert C. Owens, Jr., August J. Valenti, and Mark H. Wilcox

12. Emerging Resistance Among Candida Species: Trends and Treatment

Considerations for Candidemia 219

Ingi Lee and Theoklis Zaoutis

Part III: Interventional Strategies to Combat Resistance

13. Early Appropriate Empiric Therapy and Antimicrobial De-Escalation 231

James M. Hollands, Scott T. Micek, Peggy S. McKinnon, and Marin H. Kollef

14. Antimicrobial Cycling Programs 251

Bernard C. Camins and Victoria J. Fraser

15. Antimicrobial Stewardship: Rationale for and Practical Considerations of

Programmatic Institutional Efforts to Optimize Antimicrobial Use 261

Robert C. Owens, Jr.

16. Computer-Assisted Programs to Optimize Antimicrobial Use 301

Stanley L. Pestotnik and Jonathan B. Olson

17. Practical Application of Pharmacodynamic Principles to Optimize Therapy and

Treat Resistant Organisms: A Focus on Beta-Lactam Antibiotics 317

Thomas P. Lodise, Ben M. Lomaestro, and George L. Drusano

18. Shorter Course Antibiotic Therapy (SCAT): Principles,

Current Data, and Caveats 337

Donald E. Craven, Daniel P. McQuillen, Winnie W. Ooi, George A. Jacoby,

Efren L. Rael, and Kathleen Steger Craven

19. Impact of Guidelines on Antimicrobial Treatment of Respiratory

Tract Infections 371

Thomas M. File

20. The Impact of Hospital Epidemiology on the Management and Control of

Antimicrobial Resistance: Issues and Controversies 397

August J. Valenti

21. Combination Antimicrobial Therapy for Gram-Negative Infections:

What Is The Evidence? 433

Nasia Safdar and Cybele L. Abad

22. Antibiotic Resistance: Modern Principles and Management Strategies to Optimize

Outpatient Use of Antibiotics 461

Sharon B. Meropol and Joshua P. Metlay

Index 479

Encyclopedia of Parasitology

2008-03-30T00:29:00.004+03:00

Encyclopedia of Parasitology
by Heinz Mehlhorn (Editor)

Product Details
* Hardcover: 1573 pages
* Publisher: Springer; 3rd ed. edition (January 16, 2008)
* Language: English
* ISBN-10: 3540489940

Book Description
Knowledge in the field of parasitology must be kept at a high level and up to date in order to fight a parasitosis as quickly and effectively as possible.
The third edition of the Encyclopedia of Parasitology contributes to these goals in several ways: the number of entries has been increased by about 30%, the content has been even more improved by adding more tables and figures. The extensive linking between definitions and essays facilitates information within a minimum of time.
More than 40 international contributors, who are well known specialists in their fields, give a comprehensive review of all parasites and therapeutic strategies in veterinarian and human parasitology.
The third edition is now presented as two volumes in A-Z format and additionally in an electronic online version.

Sorry no contact table for this book but this is the most beautiful encyclopedia of parasite that I ever see.....

Plant Virology Protocols: From Viral Sequence to Protein Function

2008-03-30T00:20:00.001+03:00

Plant Virology Protocols: From Viral Sequence to Protein Function (Methods in Molecular Biology)
by Gary Foster (Editor), Elisabeth Johansen (Editor), Yiguo Hong (Editor), Peter Nagy (Editor)

Product Details
* Hardcover: 816 pages
* Publisher: Humana Press; 2nd ed. edition (March 7, 2008)
* Language: English
* ISBN-10: 1588298272

Book Description
Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.

Contents

Preface.

Contributors

Section 1 General Introduction

Chapter 1 Plant–Virus Interactions

Peter Palukaitis, John P. Carr, and James E. Schoelz

Chapter 2 Role of Capsid Proteins

John F. Bol

Chapter 3 Role of Plant Virus Movement Proteins

Michael Taliansky, Lesley Torrance, and

Natalia O. Kalinina

Chapter 4 Multiple Roles of Viral Replication Proteins in Plant

RNA Virus Replication

Peter D. Nagy and Judit Pogany

Chapter 5 Role of Silencing Suppressor Proteins

József Burgyán

Chapter 6 Role of Vector-Transmission Proteins

Véronique Ziegler-Graff and Véronique Brault

Section 2 Viral Nucleic Acid Sequence/Function Analysis

Chapter 7 In Vivo Analyses of Viral RNA Translation

William R. Staplin and W. Allen Miller

Chapter 8 In Vitro Analysis of Translation Enhancers

Aurélie M. Rakotondrafara and W. Allen Miller

Chapter 9 Identifi cation of Plant Virus IRES

Sek-Man Wong, Dora Chin-Yen Koh,

and Dingxiang Liu

Chapter 10 Analysis of Geminivirus DNA Replication

by 2-D Gel

Keith Saunders

Chapter 11 Begomoviruses: Molecular Cloning

and Identifi cation of Replication Origin

Lilian H. Florentino, Anésia A. Santos, Francisco M. Zerbini,

and Elizabeth P.B. Fontes

Chapter 12 Analysis of Viroid Replication

Ricardo Flores, María-Eugenia Gas, Diego Molina,

Carmen Hernández, and José-Antonio Daròs

Chapter 13 Biochemical Analyses of the Interactions Between

Viral Polymerases and RNAs

Young-Chan Kim and C. Cheng Kao

Chapter 14 In Situ Detection of Plant Viruses

and Virus-Specifi c Products

Andrew J. Maule and Zoltán Havelda

Chapter 15 Detection of siRNAs and miRNAs

Sakari Kauppinen and Zoltán Havelda

Chapter 16 Cloning of Short Interfering RNAs

from Virus-Infected Plants

Thien X. Ho, Rachel Rusholme, Tamas Dalmay,

and Hui Wang

Chapter 17 Solution Structure Probing of RNA

Structures

Marc R. Fabian and K. Andrew White

Chapter 18 RNA Encapsidation Assay

Padmanaban Annamalai and A.L.N. Rao

Section 3 Protein Analysis and Investigation of Protein Function

Chapter 19 Surface Plasmon Resonance Analysis

of Interactions Between Replicase Proteins of Tomato

Bushy Stunt Virus.

K.S. Rajendran and Peter D. Nagy

Chapter 20 Biochemical Approaches for Characterizing

RNA–Protein Complexes in Preparation

for High Resolution Structure Analysis

Raúl C. Gomila and Lee Gehrke

Chapter 21 Probing Interactions Between Plant Virus Movement

Proteins and Nucleic Acids

Tzvi Tzfi ra and Vitaly Citovsky

Chapter 22 Movement Profi les: A Tool for Quantitative Analysis

of Cell-to-Cell Movement of Plant Viral

Movement Proteins

Kateryna Trutnyeva, Pia Ruggenthaler,

and Elisabeth Waigmann

Chapter 23 Analysis of siRNA-Suppressor of Gene Silencing

Interactions

Lóránt Lakatos and József Burgyán

Chapter 24 Phosphorylation Analysis of Plant

Viral Proteins

Kristiina M. Mäkinen and Konstantin I. Ivanov

Chapter 25 Analysis of Interactions Between Viral Replicase

Proteins and Plant Intracellular Membranes

Hélène Sanfaçon and Guangzhi Zhang

Chapter 26 Membrane and Protein Dynamics

in Virus-Infected Plant Cells

Michael Goodin, Romit Chakrabarty,

and Sharon Yelton

Chapter 27 Site-Directed Mutagenesis of Whole

Viral Genomes

Li Liu and George P. Lomonossoff

Chapter 28 Viral Protein–Nucleic Acid Interaction:

South (North)-Western Blot

Huanting Liu

Chapter 29 Protein–Protein Interactions: The Yeast

Two-Hybrid System

Deyin Guo, Minna-Liisa Rajamäki, and Jari Valkonen

Chapter 30 NMR Analysis of Viral Protein Structures

Andrew J. Dingley, Inken Lorenzen,

and Joachim Grötzinger

Chapter 31 Localization of Viral Proteins in Plant Cells:

Protein Tagging

Sophie Haupt, Angelika Ziegler, and Lesley Torrance

Section 4 Microscopy/GFP/Protein Tagging/Infections Clones

and Other Such Tools

Chapter 32 Construction of Infectious Clones

for RNA Viruses: TMV

Sean N. Chapman

Chapter 33 Construction of Infectious cDNA Clones

for RNA Viruses: Turnip Crinkle Virus

Eugene V. Ryabov

Chapter 34 Construction of Infectious Clones

for DNA Viruses: Mastreviruses

Margaret I. Boulton

Chapter 35 Construction of Infectious Clones of Double-Stranded

DNA Viruses of Plants Using Citrus Yellow

Mosaic Virus as an Example

Qi Huang and John S. Hartung

Chapter 36 Insertion of Introns: A Strategy to Facilitate

Assembly of Infectious Full Length Clones

I. Elisabeth Johansen and Ole Søgaard Lund

Chapter 37 Analysis of Cell-to-Cell and Long-Distance Movement

of Apple Latent Spherical Virus in Infected Plants Using

Green, Cyan, and Yellow Fluorescent Proteins

Tsubasa Takahashi and Nobuyuki Yoshikawa

Chapter 38 Agroinoculation: A Simple Procedure for Systemic

Infection of Plants with Viruses

Zarir E. Vaghchhipawala and Kirankumar S. Mysore

Chapter 39 Geminivirus: Biolistic Inoculation

and Molecular Diagnosis

Anésia A. Santos, Lilian H. Florentino,

Acássia B.L. Pires, and Elizabeth P.B. Fontes

Section 5 Genomics, Host Factors and Plant Based Studies

Chapter 40 Expression Microarrays in Plant–Virus

Interaction

Kristina Gruden, Maruša Pompe-Novak, Špela Baebler,

Hana Krecˇicˇ-Stres, Nataša Toplak, Matjaž Hren, Polona Kogovšek,

Lisa Gow, Gary D. Foster, Neil Boonham, and Maja Ravnikar

Chapter 41 Genome-Wide Screens for Identifi cation of Host

Factors in Viral Replication

Tadas Panavas, Elena Serviene, Judit Pogany,

and Peter D. Nagy

Chapter 42 Phosphorylation of Movement Proteins

by the Plasmodesmal-Associated Protein Kinase

Jung-Youn Lee

Chapter 43 Virus-Induced Gene Silencing as a Tool

to Identify Host Genes Affecting Viral Pathogenicity

Xiaohong Zhu and S.P. Dinesh-Kumar

Chapter 44 Yeast Two-Hybrid Assay to Identify

Host–Virus Interactions

Stuart A. MacFarlane and Joachim F. Uhrig

Index

Parasitism and Ecosystems

2008-03-29T22:47:00.000+03:00

Parasitism and Ecosystems
by Frederic Thomas (Editor), Francois Renaud (Editor), Jean-Francois Guegan (Editor)

Product Details
* Paperback: 232 pages
* Publisher: Oxford University Press, USA (March 3, 2005)
* Language: English
* ISBN-10: 0198529872

Book Description
For several years there has been a growing interest in understanding the dynamics of parasites in ecosystems, as well as the diversity of ways in which they influence ecosystem functioning through their effects on host populations and communities. Ecologists, epidemiologists, evolutionary
biologists, and other scientists are increasingly coming to realise that parasites must be taken into account when studying ecosystems. Parasitism and Ecosystems summarizes current knowledge on this topic, providing a comprehensive overview for researchers and students. It represents the first
synthesis of both the roles and the consequences of pathogens in ecosystems, utilizing well-documented case-studies to illustrate the main issues as well as identifying prospects for future research.

Contents

Contributors ix

Introduction—Parasites, diversity, and the ecosystem 1

Peter Hudson

1 Linking ecosystem and parasite ecology 13

Michel Loreau, Jacques Roy, and David Tilman

2 Are there general laws in parasite community

ecology? The emergence of spatial parasitology

and epidemiology 22

J.-F. Guégan, S. Morand, and R. Poulin

3 Parasitism and the regulation of host populations 43

Anders Pape Mّller

4 Food web patterns and the parasite’s perspective 54

Michael V.K. Sukhdeo and Alexander D. Hernandez

5 Ecosystems and parasitism: the spatial dimension 68

Robert Holt and Thierry Boulinier

6 Parasitism and hostile environments 85

Richard C. Tinsley

7 Parasitism and environmental disturbances 113

Kevin D. Lafferty and Armand M. Kuris

8 Parasitism, biodiversity, and conservation 124

Frédéric Thomas, Michael B. Bonsall, and Andy P. Dobson

9 Subverting hosts and diverting ecosystems: an

evolutionary modelling perspective 140

Sam P. Brown, Jean-Baptiste André, Jean-Baptiste

Ferdy, and Bernard Godelle

10 Parasitism in man-made ecosystems 155

François Renaud, Thierry De Meeüs, and Andrew F. Read

Conclusion—Parasites, communities, and ecosystems:

conclusions and perspectives 171

Gary G. Mittelbach

References 177

Index 217

A-Z of Haematology

2008-03-29T22:42:00.002+03:00

Product Details

Paperback: 248 pages
Publisher: Blackwell Publishing (Nov 2002)
Language English
ISBN-10: 1405103221
ISBN-13: 978-1405103220
Product Dimensions: 23 x 15.6 x 1.6 cm

Book Description:
"Very little of substance is missing in this remarkable book. We suspect that it will be dog-eared and worn with good use in any laboratory or clinic." British Journal of Haematology "A great little book that will help all those with the difficult, hard to remember terms in haematology - ideal for trainees." Drew Provan, Consultant Haematologist, Royal London Hospital "Good, quick and easy reference guide - Barbara's books have an excellent reputation amongst haematologists around the world." Mike Murphy, Consultant Haematologist, John Radcliffe Hospital.

Contents

Preface, vii

Online Resources, ix

A–Z of Haematology, 1

Atlas of Immunology, Second Edition

2008-03-29T22:37:00.001+03:00

Product Details
»Book Publisher: CRC (29 December, 2003)
»ISBN: 0849315670
»Book author: Julius M. Cruse, Robert E. Lewis

Book Description:

With more than 1,250 computer-generated figures, line drawings, and photographs, the Atlas of Immunology clearly demonstrates that “a picture is worth a thousand words.” Written for students, basic scientists, and clinicians, this second edition provides a thorough and up-to-date treatment of all the concepts needed to comprehend contemporary immunology. The subject matter ranges from photographs of historical figures to molecular structures of recently characterized cytokines, major histocompatibility complex molecules, immunoglobins, and molecules of related interest.

Table of Contents

Chapter 1

History of Immunology

Chapter 2

Molecules, Cells, and Tissues of the Immune Response

Chapter 3

Antigens and Immunogens

Chapter 4

Major Histocompatibility Complex

Chapter 5

Antigen Processing and Presentation

Chapter 6

B Lymphocyte Development and Immunoglobulin Genes

Chapter 7

Immunoglobulin Synthesis, Properties, Structure, and Function

Chapter 8

Antigen–Antibody Interactions

Chapter 9

The Thymus and T Lymphocytes

Chapter 10

Cytokines and Chemokines

Chapter 11

The Complement System

Chapter 12

Types I, II, III, and IV Hypersensitivity

Chapter 13

Immunoregulation and Immunologic Tolerance

Chapter 14

Autoimmunity

Chapter 15

Mucosal Immunity

Chapter 16

Immunohematology

Chapter 17

Immunological Diseases and Immunopathology

Chapter 18

Immunodeficiencies: Congenital and Acquired

Chapter 19

Acquired Immune Deficiency Syndrome (AIDS)

Chapter 20

Immunosuppression

Chapter 21

Transplantation Immunology

Chapter 22

Tumor Immunology

Chapter 23

Immunity against Microorganisms

Chapter 24

Vaccines and Immunization

Chapter 25

Therapeutic Immunology

Chapter 26

Comparative Immunology

Chapter 27

Immunological Methods and Molecular Techniques

Chapter 28

Diagnostic Immunohistochemistry

Handbook of Media for Environmental Microbiology

2008-03-29T22:27:00.002+03:00

Product Details

Hardcover: 672 pages
Publisher: CRC Press Inc; 2Rev Ed edition (29 Mar 2005)
Language English
ISBN-10: 0849335604
ISBN-13: 978-0849335600
Product Dimensions: 25.1 x 17.8 x 4.1 cm

Book Description:
The second edition of a bestseller, this book provides a comprehensive reference for the cultivation of bacteria, Archaea, and fungi from diverse environments, including extreme habitats. Expanded to include 2,000 media formulations, this book compiles the descriptions of media of relevance for the cultivation of microorganisms from soil, water, and air. The format allows easy reference to the information needed to prepare media for the cultivation of microorganisms required for environmental analysis, including the determination of water safety. The media are organized alphabetically, and each listing includes medium composition, instructions for preparation, commercial sources, and uses.

Contents

INTRODUCTION

Overview

Organization

Names of Media

Trademarks

Composition of Media

Agars

Peptones

Meat and Plant Extracts

Growth Factors

Selective Components

Differential Components

pH Buffers

Preparation of Media

Tyndallization

Inspissation.

Autoclaving

Filtration

Caution about Hazardous Components

Uses of Media

References

Web Resources

MEDIA, ALPHABETICAL

INDEX