New Antibiotic Targets (Methods in Molecular Medicine)
By W. Scott Champney
Product Details
* Hardcover: 296 pages
* Publisher: Humana Press; 1 edition (January 15, 2008)
* Language: English
* ISBN-10: 1588299155
Book Description
A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In New Antibiotic Targets, W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies.
New Antibiotic Targets provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.
Contents
Preface .
Contributors
1 Biocomputational Strategies for Microbial Drug Target Identification
Kishore R. Sakharkar, Meena K. Sakharkar,
and Vincent T. K. Chow
2 Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase
IV Activities
L. Mark Fisher and Xiao-Su Pan
3 A Method to Assay Inhibitors of DNA Polymerase IIIC Activity
Michelle M. Butler and George E. Wright
4 Methods to Identify and Characterize Inhibitors of Bacterial RNA
Polymerase
A. Simon Lynch and Qun Du
5 Methods to Assay Inhibitors of tRNA Synthetase Activity
Dieter Beyer, Hein Peter Kroll, and Heike Brِtz-Oesterhelt
6 Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly
W. Scott Champney
7 Inhibition of Chaperone-Dependent Bacterial Ribosome Biogenesis
Abdalla Al Refaii and Jean-Hervé Alix
8 Assays for the Identification of Inhibitors Targeting Specific
Translational Steps
Letizia Brandi, John Dresios, and Claudio O. Gualerzi
9 SPARK: A New Peptidyl Transferase Activity Assay
Alexander S. Mankin and Norbert Polacek
10 High-Throughput Screening of Peptide Deformylase Inhibitors
Kiet T. Nguyen and Dehua Pei
11 A Method to Assay Penicillin-Binding Proteins
Michael J. Pucci and Thomas J. Dougherty
12 A Method to Assay Inhibitors of Lipopolysaccharide Synthesis
Marcy Hernick and Carol A. Fierke .
13 Methods for Assessing the Structure and Function of Cationic
Antimicrobial Peptides
Michelle Pate and Jack Blazyk
14 Flow Cytometry of Bacterial Membrane Potential and Permeability
Howard M. Shapiro
15 Bacterial Efflux Pump Inhibitors
Barbara J. Kamicker, Michael T. Sweeney, Frank Kaczmarek,
Fadia Dib-Hajj, Wenchi Shang, Kim Crimin, Joan Duignan,
and Thomas D. Gootz
16 Mycobacterium tuberculosis _-Ketoacyl Acyl Carrier Protein
Synthase III (mtFabH) Assay: Principles and Method
Sarbjot Sachdeva and Kevin A. Reynolds
17 Screening for Compounds That Affect the Interaction Between
Bacterial Two-Component Single Transduction Response
Regulator Protein and Cognate Promoter DNA
Matthew G. Erickson, Andrew T. Ulijasz, and Bernard Weisblum
18 The Activity of rRNA Resistance Methyltransferases Assessed
by MALDI Mass Spectrometry
Stephen Douthwaite, Rikke Lind Jensen, and Finn Kirpekar
19 Assays for _-Lactamase Activity and Inhibition
Thammaiah Viswanatha, Laura Marrone, Valerie Goodfellow,
and Gary I. Dmitrienko
20 Studies of Enzymes That Cause Resistance to Aminoglycoside
Antibiotics
Engin H. Serpersu, Can , and Edward Wright
